— Mutation testing was initially proposed in the 1970s as intends to guarantee vigour in test case improvement. By making syntactically right substitutions inside the software under test (SUT) and rehashing the test execution stage against the adjusted code, an evaluation could be made of test quality contingent upon if the definitive test cases could locate the code adjustment. Mutation testing is normally used in small code programs, but for a small portion of large program or for a specific code it is used. This paper test a access control part of web based applications for mutant testing, till date testing of web based application is only up to application level only. Generally test cases are executed and stop the testing, but we can not check capability of test cases.