Image Processing refers to the use of algorithm to perform processing on digital image. Microscopic images like some microorganism images contain different type of noises which reduce the quality of the images. Removing noise is a difficult task. Noise removal is an issue of image processing. Images containing noise degrade the quality of the images. Removing noise is an important processing task. After removing noise from the images, the visual effect will not be proper. This paper presents an approach to de-noise based on averaging of pixels in 5X5 window and wavelets is proposed.